目的 探讨异丙酚对胶质瘤U87细胞增殖、侵袭、迁移及Janus激酶2/信号转导子和转录激活子3(JAK2/STAT3)通路的影响。方法 采用MTT法测定不同浓度的异丙酚对胶质瘤U87细胞和人正常星型胶质细胞HEB生长抑制作用,Transwell侵袭实验测定2、5、10 μM异丙酚对胶质瘤U87细胞体外侵袭能力的影响,划痕实验测定2、5、10 μM异丙酚对胶质瘤U87细胞体外迁移能力的影响,蛋白印迹法(WB)测定10 μM异丙酚对胶质瘤U87细胞JAK2/STAT3通路的影响。结果 与对照组相比,5、10、25、50、100 μM异丙酚均能显著抑制胶质瘤U87细胞增殖,差异有统计学意义(P<0.05),后续选择2、5、10 μM异丙酚进行实验。与对照组相比,胶质瘤U87细胞经2、5、10 μM异丙酚处理后,侵袭能力显著降低,差异有统计学意义(P<0.05)。细胞划痕实验说明,胶质瘤U87细胞经2、5、10 μM异丙酚处理48 h后,迁移能力分别降低了(19.69±2.67)%、(41.41±3.28)%、(59.75±2.91)%,与对照组相比差异有统计学意义(P<0.05)。且胶质瘤U87细胞中JAK2蛋白磷酸化水平、STAT3蛋白磷酸化水平明显下调,与对照组相比差异有统计学意义(P<0.05)。结论 异丙酚可能通过下调JAK2/STAT3信号传导通路相关蛋白表达,抑制胶质瘤U87细胞增殖、侵袭、迁移能力。
Abstract
Objective To study the effects of propofol on the proliferation, invasion, migration and Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway of glioma U87 cells. Methods MTT assay was used to determine the inhibitory effect of propofol at different concentrations on the growth of glioma U87 cells and human normal astrocyte HEB. Transwell invasion assay was used to determine the effects of 2 μM, 5 μM and 10 μM propofol on the invasive ability of glioma U87 cells in vitro. Scratch assay was used to determine the effects of 2 μM, 5 μM and 10 μM propofol on migration of glioma U87 cells in vitro, and the effect of propofol on JAK2/STAT3 pathway in U87 glioma cells was determined by Western blotting (WB). Results Compared with the control group, 5 μM, 10 μM, 25 μM, 50 μM and 100 μM propofol could significantly inhibit the proliferation of glioma U87 cells (P<0.05). Follow-up experiments were conducted with 2 μM, 5 μM and 10 μM propofol. Compared with the control group, the invasive ability of glioma U87 cells treated with 2 μM, 5 μM and 10 μM propofol decreased significantly (P<0.05). Scratch assay showed that the migration ability of U87 glioma cells treated with 2 μM, 5 μM and 10 μM propofol for 48 hours decreased by (19.69±2.67)%, (41.41±3.28)% and (59.75±2.91)% respectively, and there was significant difference between the two groups (P<0.05). The levels of JAK2 protein phosphorylation and STAT3 protein phosphorylation in U87 glioma cells treated with 2 μM, 5 μM and 10 μM propofol for 48 hours were significantly lower than those of the control group. Conclusions Propofol may inhibit the proliferation, invasion and migration of glioma U87 cells by down-regulating the expression of JAK2/STAT3 signal transduction pathway-related proteins.
关键词
异丙酚 /
胶质瘤 /
细胞增殖 /
细胞侵袭 /
细胞迁移
Key words
propofol /
glioma /
cell proliferation /
cell invasion /
cell migration
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