抗骨髓炎片对骨感染家兔血清细胞因子的影响

骞秀芳; 羿凤云; 孙会仙; 王鲁君

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武警医学 ›› 2022, Vol. 33 ›› Issue (1) : 17-21.

论著

抗骨髓炎片对骨感染家兔血清细胞因子的影响

骞秀芳¹, 羿凤云¹, 孙会仙¹, 王鲁君²

作者信息 +

Effect of Kanggusuiyan tablets on serum cytokines in rabbits with bone infections

QIAN Xiufang¹, YI Fengyun¹, SUN Huixian¹, Wang Lujun²

Author information +

文章历史 +

摘要

目的观察抗骨髓炎片对慢性骨髓炎(chronic osteomyelitis,COL)家兔血清中细胞因子的影响。方法采用右胫骨骨髓腔内金黄色葡萄球菌注射法制备家兔COL模型,按随机数字表法分为假手术组、模型组、阳性药克林霉素组(60 mg/kg)、抗骨髓炎片高剂量组(480 mg/kg)、抗骨髓炎片低剂量组(240 mg/kg)。在灌胃给药后第2、4、6周检测右胫骨X线片,同时给予诊断分级,末次给药后取血测定红细胞沉降率(ESR)和白细胞计数(WBC),分离血清测定降钙素原(PCT)、C反应蛋白(CRP)及细胞因子白介素-1β(IL-1β)、IL-4、IL-6、IL-10等水平。结果与模型组同时间点相比,抗骨髓炎片高剂量组第2、4、6周及低剂量组第4、6周的X线片诊断分级,差异均有统计学意义(P<0.05)。抗骨髓炎片高、低剂量组ESR、WBC、PCT和CRP在给药后第2、4、6周与模型组同时间点相比均有明显降低,差异有统计学意义(P<0.05)。与模型组同时间点相比,抗骨髓炎片高、低剂量组第2、4、6周的促炎细胞因子IL-1β和IL-6含量均有显著降低(P<0.05),第6周抗炎细胞因子IL-4和IL-10含量则有显著升高,差异有统计学意义(P<0.01)。结论抗骨髓炎片通过恢复促炎/抗炎细胞因子平衡、缓解炎性反应等环节实现对COL的治疗作用。

Abstract

Objective To observe the effect of Kanggusuiyan tablets on serum cytokines in rabbits with chronic osteomyelitis (COL). Methods Rabbits received an injection of Staphylococcus aureus intramedullarly in right tibias to induce an experimental model of COL before being divided into five groups using the random number table method: sham, model, positive control (clindamycin60 mg/kg), Kanggusuiyan tablet high and low dose (480 and 240 mg/kg) groups. Animals were orally administrated for six weeks. On the 2nd, 4th and 6th week after administration, the right tibias of rabbits were examined by X-ray and their severity was graded, while the erythrocyte sedimentation rate (ESR) and white blood cells (WBCs) were calculated. The expressions of procalcitonin (PCT), C-reactive protein (CRP), interleukin-1β (IL-1β), IL-4, IL-6 and IL-10 in sera were measured by enzyme-linked immunosorbent assay. Results The severity of the right tibia in Kanggusuiyan tablet groups (480 mg/kg on the 2nd, 4th and 6th week and 240 mg/kg on the 4th and 6th week, respectively) was markedly attenuated compared to the model group (P<0.05 or P<0.01). In comparison with the model group, levels of ESR, WBC, PCT and CRP in Kanggusuiyan tablet groups were significantly reduced on the 2nd, 4th and 6th week, respectively (P<0.05 or P<0.01). Compared to the model group, the serum levels of IL-1β and IL-6 in Kanggusuiyan tablet groups were markedly decreased on the 2nd, 4th and 6th week, respectively (P<0.05 or P<0.01), while the expressions of IL-4 and IL-10 were markedly increased on the 6th week (P<0.01). Conclusions Kanggusuiyan tablets could protect against COL in rabbits. The mechanism likely involves the modulation of proinflammatory/anti-inflammatory cytokine balance and improvement of inflammatory responses.

导出引用

骞秀芳, 羿凤云, 孙会仙, 王鲁君. 抗骨髓炎片对骨感染家兔血清细胞因子的影响[J]. 武警医学. 2022, 33(1): 17-21

QIAN Xiufang, YI Fengyun, SUN Huixian, Wang Lujun. Effect of Kanggusuiyan tablets on serum cytokines in rabbits with bone infections[J]. Medical Journal of the Chinese People Armed Police Forces. 2022, 33(1): 17-21

中图分类号： R962.2

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