目的 探讨白三烯B4受体(BLT1和BLT2)在寻常型银屑病患者皮损组织中的表达及与银屑病发病的相关性。方法 选取2014-11至2018-10解放军总医院第三医学中心皮肤科治疗的寻常型银屑病30例患者的石蜡组织标本为银屑病组,另选取整形外科手术切除的正常皮肤30例为对照组。采用链霉素-生物素过氧化物酶(S-P)三步法分别检测两组皮肤表皮中BLT1、BLT2的表达,使用Image-Pro Plus6.0软件系统测量每个视野阳性染色区域的平均光密度(AIOD)值。分析银屑病患者皮损与正常皮肤表皮中BLT1、BLT2的表达强度差异。结果 BLT1、BLT2在寻常型银屑病皮损组织表皮中主要表达于棘层,在正常皮肤组织中主要表达于基底层。与正常皮肤相比,寻常型银屑病患者皮损表皮中BLT1、BLT2表达均明显增强,差异有统计学意义(P<0.05)。结论 白三烯B4受体BLT1、BLT2在寻常型银屑病皮损组织表皮棘层中高表达,提示二者与银屑病的表皮过度增殖有相关性。
Abstract
Objective To detect the expressions of leukotriene B4 receptors in epidermis of psoriasis vulgaris and study the correlations between BLT1, BLT2 and the pathogenesis of psoriasis. Methods Thirty specimens of paraffin tissue of psoriasis vulgaris treated in the Department of Dermatology of the former Armed Police General Hospital between November 2014 to October 2018 were collected as the patient group, while another 30 normal skin specimens removed by plastic surgery were collected as the control group.The expressions of BLT1 and BLT2 in psoriasis patients and normal skin epidermis were detected using the three-step streptomycin-biotin peroxidase (S-P) method, while the average integrated optical density (AIOD) of a positive staining area in each visual field was measured by image-Pro Plus6.0 software system.Independent sample T test was used to analyze the difference in the BLT1 and BLT2 expression intensity between psoriasis patients and normal skin. Results BLT1 and BLT2 were mainly expressed in the spinous layer in the epidermis of psoriasis vulgaris, but in the basal layer of normal skin.Compared with normal skin, the expressions of BLT1 and BLT2 in psoriasis vulgaris epidermis were significantly increased (P<0.05). Conclusions Leukotriene B4 receptors BLT1 and BLT2 are highly expressed in the epidermal spinous layer of psoriasis vulgaris, suggesting that the two receptors may be related to the epidermal hyperproliferation of psoriasis vulgaris.
关键词
寻常型银屑病 /
白三烯B4受体 /
免疫组织化学
Key words
psoriasis vulgaris /
leukotriene B4 receptor /
immunohistochemistry
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参考文献
[1] Ikai K. Psoriasis and the arachidonic acid cascade[J]. J of Dermatological Sci, 1999, 21(3):135-146.
[2] Capdevila J H, Falck J R. The arachidonic acid monooxygenase. from biochemical curiosity to physiological/pathophysiological significance[J]. J Lipid Res, 2018, 59(11):2047-2062.
[3] 宋秀风,陈 楠,刘雯敏,等.白三烯,IL-8和TNF-α在银屑病患者血,尿和皮损中的表达[J].中国麻风皮肤病杂志,2011,27(5):314-316.
[4] Yu S, Tetsuya H, Satoshi N, et al. Resolvin E1 attenuates murine psoriatic dermatitis[J]. Sci Rep, 2018, 8(1):11873.
[5] Ibrahim M A, Hassan A M. Comparative modeling and evaluation of leukotriene b4 receptors for selective drug discovery towards the treatment of inflammatory diseases[J]. Molecular and Cell Biology,2018,37(6):518-530.
[6] Teru K I, Dainic H I, Akihiko, et al. The epithelial immune microenvironment (EIME) in atopic dermatitis and psoriasis[J]. Nature Immunology, 2018, 19(12):1286-1298.
[7] 张建中 译.皮肤病理学[M].2版. 天津:天津科技翻译出版有限公司, 2017:150-160.
[8] Katayama H. Development of psoriasis by continuous neutrophil infiltration into the epidermis[J]. Katayama H. Exp Dermatol,2018,27(10):1084-1091.
[9] Hirakata T, Matsuda A, Yokomizo T. Leukotriene B4 receptors as therapeutic targets for ophthalmic diseases[J]. BBA Mol Cell Biol Lipid, 2020, 1865(9):158756.
[10] BenhadouF,Glitzner E, Brisebarre A, et al. Epidermal autonomous VEGFA/Flt1/Nrp1 functions mediate psoriasis-like disease[J]. Sci Adv, 2020, 6(2): 5849.
[11] Fumiyuki S, Takehiko Y. The leukotriene receptors as therapeutic targets of inflammatory diseases[J]. Int Immunol, 2019, 31(9):607-615.
[12] Saeki K,Yokomizo T. Identification, signaling, and functions of LTB4 receptors[J]. Semin Immunol, 2017, 33:30-36.
[13] Sumida H,Yanagida K, Kita Y, et al. Interplay between CXCR2 and BLT1 facilitates neutrophil infiltration and resultant keratinocyte activation in a murine model of imiquimod-induced psoriasis[J].J Immunol, 2014, 192(9):4361-4369.
[14] 马 聪, 刘永霞, 韩春光,等. LTB4-BLT2信号通路对小鼠树突状细胞功能的影响[J]. 军事医学, 2013(3):171-175.
[15] 张 瑛, 郝 飞, 钟白玉,等. 白三烯B4受体在表皮肿瘤中的表达[J]. 临床皮肤科杂志, 2008, 37(1):20-22.
[16] 刘 芳. 银屑病瘙痒特征及其发生机制的初步研究[D]. 合肥:安徽医科大学, 2014.
基金
北京市海淀区卫生健康发展科研培育计划(HP2021-50-80403)
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