目的 探究黄芩对妊娠晚期大鼠子宫运动节律及对前列腺素E(PGE)、环氧合酶2(COX2)水平的影响。方法 100只健康成年SPF级雌性大鼠、50只雄性大鼠适应性饲养7 d后,按照2∶1雌雄合笼交配,次日用无菌棉签给雌鼠作阴道涂片并进行镜检判断是否妊娠。取成功妊娠的40只大鼠,按体重排序随机分组法分为对照组及黄芩低、中、高剂量组,各10只。黄芩低、中、高剂量组于妊娠第19天时给予成人临床等效剂量的4、8、16倍(400、800、1600 mg/kg)灌胃(1次/d),对照组同时间给予等量生理盐水灌胃,当日观察大鼠胎鼠娩出情况;于妊娠第21天时,张力传感器测定大鼠子宫活动力、宫缩强度及宫缩频率;实时荧光定量PCR法、免疫印迹法分别检测大鼠子宫肌条组织中PGE、COX2 mRNA、蛋白水平。结果 黄芩低、中、高剂量组娩出鼠数分别为36、62、87只,较对照组(娩出0只)多;宫缩频率分别为(3.57±0.53)、(5.82±0.87)、(5.82±0.87)次/min,较对照组的(2.11±0.31)次/min高;宫缩强度分别为(1.97±0.29)、(2.58±0.38)、(3.26±0.50)g,较对照组的(1.06±0.23)g高;子宫活动力分别为(6.85±1.03)、(15.03±2.26)、(25.07±3.76)g/(次·min),较对照组(2.25±0.33)g/(次·min)高;子宫肌条组织中PGE mRNA分别为1.26±0.19、1.58±0.23、1.76±0.25,较对照组(1.00±0.15)升高;COX2 mRNA分别为1.33±0.19、1.62±0.25、1.83±0.30,较对照组(1.01±0.16)高;PGE蛋白分别为0.59±0.05、0.86±0.12、1.15±0.17,较对照组的(0.27±0.05)高;COX2蛋白分别为0.60±0.09、0.93±0.14、1.27±0.19,较对照组(0.37±0.06)高;上述差异均有统计学意义(均P<0.05)。结论 黄芩可能通过促进妊娠晚期大鼠子宫肌组织COX2及PGE合成及分泌,促进妊娠晚期子宫收缩进而促进分娩。
Abstract
Objective To explore the effect of Scutellaria baicalensis on the uterine movement rhythm and levels of prostaglandin E (PGE) and cyclooxygenase 2 (COX2) in rats in the third trimester of pregnancy.Methods One hundred healthy adult SPF female rats and 50 male rats were adaptively reared for 7 days before mating in a 2∶1 male-female co-cage. The next day, female mice were smeared with sterile cotton swabs and examined by microscopy to find out whether they were pregnant. The forty pregnant rats were randomly divided into the control group and Scutellaria baicalensis low, middle and high dose groups in order of body weight, with 10 rats in each group. In the low, middle and high dose groups of Scutellaria baicalensis, doses that were 4,8 and 16 times (400 mg/kg, 800 mg/kg, 1600 mg/kg) the clinical equivalent dose for adults were given to the rats at the 19th day of pregnancy (once a day), while the control group was given the same dose of normal saline by gavage at the same time. The delivery of rat fetuses was observed on the same day. On the 21st day of pregnancy, the uterine activity, intensity of contractions and frequency of contractions in rats were recorded. The levels of PGE and COX2 mRNA and protein were detected by real-time PCR and Western blotting respectively.Results In the low, medium and high dose groups of Scutellaria baicalensis, the number of rats delivered was 36, 62 and 87 respectively, but was zero in the control group. The frequency of uterine contractions was (3.57±0.53, 5.82±0.87 and 5.82±0.87) times/min respectively, which was higher than that of the control group (2.11±0.31) times/min. The intensity of uterine contractions was (1.97±0.29, 2.58±0.38 and 3.26±0.50) g respectively, which was higher than that of the control group (1.06±0.23) g. The uterine activities were (6.85±1.03, 15.03±2.26 and 25.07±3.76) g/(time·min) respectively, which were higher than those of the control group (2.25±0.33) g/(time·min). The PGE mRNA levels in uterine muscle strip tissues were (1.26±0.19, 1.58±0.23 and 1.76±0.25) respectively, which were higher than in the control group (1.00±0.15). The COX2 mRNA levels were (1.33±0.19, 1.62±0.25 and 1.83±0.30) respectively, higher than those of the control group (1.01±0.16). The PGE protein levels were (0.59±0.05, 0.86±0.12 and 1.15±0.17) respectively, which were higher than those of the control group (0.27±0.05). The COX2 protein levels were (0.60±0.09, 0.93±0.14, 1.27±0.19) respectively, which were higher than those of the control group (0.37±0.06), and the above differences were all statistically significant (all P<0.05).Conclusions Scutellaria baicalensis may promote the synthesis and secretion of COX2 and PGE in uterine muscle tissues of rats during late pregnancy.
关键词
妊娠晚期 /
黄芩 /
子宫运动节律 /
前列腺素E /
环氧合酶-2
Key words
late pregnancy /
Scutellaria baicalensis /
uterine movement rhythm /
prostaglandin e /
cyclooxygenase-2
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基金
2017年沈阳市科技计划项目(17-230-9-10)
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