目的 探讨白藜芦醇通过MyD88/TLR4/NF-kB信号通路影响结肠癌细胞凋亡的机制。方法 选取不同剂量白藜芦醇处理24 h后的结肠癌细胞,TUNEL法检测细胞凋亡率的变化,利用RT-PCR和Western blot检测白藜芦醇处理后结肠癌细胞MyD88/TLR4/NF-kB信号通路中关键信号分子的表达变化。结果 中高剂量白藜芦醇处理结肠癌细胞系HT-29、sw480和 LoVo后,细胞凋亡率显著上升;Bcl-2蛋白和mRNA含量表达下降,Bax、cleaved-Caspase 3蛋白和mRNA含量表达显著升高;细胞上清中VEGF蛋白表达明显降低;细胞中MyD88、TLR4、NF-kB和mRNA含量均明显降低。结论 白藜芦醇可能通过MyD88/TLR4/NF-kB信号通路促进结肠癌细胞凋亡从而抑制肿瘤发生发展。
Abstract
Objective To investigate the mechanism of apoptosis of colon cancer cells induced by resveratrol through MyD88/TLR4/ NF-KB signaling pathway.Methods With different doses of resveratrol for 24 h, TUNEL assay was used to detect the apoptosis rate of colon cancer cells and Western blot and RT-PCR were employed to test the expression of key signal molecules in MyD88/TLR4/NF-kB signaling pathway.Results The apoptosis rates of HT-29, SW480 and LoVo significantly increased after treatment. The protein and mRNA levels of Bax and Cleaved Caspase 3 significantly increased and Bcl-2 was downregulated. The expression of VEGF protein in cell supernatant decreased significantly. MyD88, TLR4 and NF-KB were significantly down regulated (P<0.05).Conclusions Resveratrol may promote the apoptosis of colon cancer cells through MyD88/TLR4/ NF-KB signaling pathway to inhibit the development of tumor.
关键词
白藜芦醇 /
结肠癌 /
MyD88 /
TLR4 /
NF-kB
Key words
resveratrol /
colon cancer /
MyD88 /
TLR4 /
NF-kB
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