大麻二酚对高糖诱导小鼠肾脏足细胞系MPC5细胞凋亡的保护作用

苏静华; 焦婷; 韩宁; 苏静克; 党瑞杰; 李琳; 张海松

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武警医学 ›› 2022, Vol. 33 ›› Issue (4) : 303-306.

论著

大麻二酚对高糖诱导小鼠肾脏足细胞系MPC5细胞凋亡的保护作用

苏静华¹, 焦婷², 韩宁³, 苏静克³, 党瑞杰⁴, 李琳⁴, 张海松²

作者信息 +

Protective effect of cannabidiol on high glucose-induced podocytes MPC5 apoptosis

SU Jinghua¹, JIAO Ting², HAN Ning³, SU Jingke³, DANG Ruijie⁴, LI Lin⁴, ZHANG Haisong²

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文章历史 +

摘要

目的探讨大麻二酚(cannabidiol, CBD)对高糖环境中小鼠足细胞系MPC5细胞凋亡的影响。方法将MPC5细胞分为对照组(Vehicle)、高糖组(high glucose, HG)和CBD组。CCK-8试剂盒检测MPC5细胞的增殖活性,流式细胞术检测MPC5细胞的凋亡率,Western blot检测裂孔膜蛋白Nephrin与凋亡相关蛋白Bax及Bcl-2的表达情况。结果与Vehicle组相比,HG组明显抑制MPC5增殖并促进其凋亡[(25.27±2.15)% vs. (8.43±0.70) %];而CBD干预显著促进高糖环境中MPC5增殖且抑制凋亡[(12.83±1.05)% vs. (25.27±2.15) %; P＜0.05]。同时,CBD干预显著升高高糖环境中MPC5细胞Nephrin蛋白的表达[(0.77±0.06) vs. (0.32±0.10)],降低促凋亡蛋白Bax与抗凋亡蛋白Bcl-2的蛋白表达比[(1.02±0.09) vs. (12.30±0.09)]。结论 CBD能缓解高糖诱导的MPC5细胞凋亡。

Abstract

Objective To investigate the effect of cannabidiol (CBD) on podocytes MPC5 apoptosis induced by high glucose.Methods MPC5 cells were randomly divided into vehicle group, high glucose (HG) group and CBD group. CCK-8 method was used to access cell proliferation, flow cytometry was used to evaluate apoptotic rate, and western blot was used to investigate protein level of Nephrin and apoptosis-related proteins Bax and Bcl-2.Results Compared with the Vehicle group, HG significantly inhibited MPC5 proliferation and promoted MPC5 apoptosis [(25.27±2.15)% vs. (8.43±0.70)%], while CBD obviously promoted MPC5 proliferation and inhibited MPC5 apoptosis [(12.83±1.05)% vs. (25.27±2.15)%; P＜0.05] in the high glucose environment. Meanwhile, CBD administration increased Nephrin expression [(0.77±0.06) vs. (0.32±0.10)], and reduced Bax/Bcl-2 [(1.02±0.09) vs. (12.30±0.09); P＜0.05].Conclusions CBD attenuates high glucose-induced MPC5 apoptosis.

导出引用

苏静华, 焦婷, 韩宁, 苏静克, 党瑞杰, 李琳, 张海松. 大麻二酚对高糖诱导小鼠肾脏足细胞系MPC5细胞凋亡的保护作用[J]. 武警医学. 2022, 33(4): 303-306

SU Jinghua, JIAO Ting, HAN Ning, SU Jingke, DANG Ruijie, LI Lin, ZHANG Haisong. Protective effect of cannabidiol on high glucose-induced podocytes MPC5 apoptosis[J]. Medical Journal of the Chinese People Armed Police Forces. 2022, 33(4): 303-306

中图分类号： R285.5

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