目的 探讨ATP结合盒转运体A1 (ABCA1)在卵巢癌(ovarian cancer)中的表达及临床意义。方法 选择2016-01至2020-12首都医科大学附属北京妇产医院患者212例,其中106例卵巢癌患者为卵巢癌组,另106例良性卵巢上皮性肿瘤患者为对照组。常规收集患者空腹肘静脉血,用酶氧化法分析两组之间血脂含量[三酰甘油(CHO)、总胆固醇(TG)、高密度脂蛋白(HDL)及低密度脂蛋白(LDL)]并进行差异分析;采用免疫组化及Western-blot检测组织中ABCA1的表达情况并进行差异分析;采用Kaplan-Meier生存分析方法估计ABCA1表达情况及卵巢癌患者生存之间的关系,并应用Log-rank方法进一步验证。结果 与对照组相比,卵巢癌组患者外周血中血脂水平(CHO、TG、HDL、LDL)显著降低(P<0.01); ABCA1在两组中均存在表达,而卵巢癌组的表达量(0.15±0.02)明显高于对照组(0.06±0.01),差异有统计学意义(P<0.01);生存分析结果显示,ABCA1阳性表达组的中位总生存期为48.0个月(95%CI: 45.9~51.5个月),而ABCA1阴性表达组的中位总生存期为57.0个月(95%CI: 54.7~58.9个月),ABCA1表达量与卵巢癌患者生存期之间存在负相关(P<0.01)。结论 ABCA1与卵巢癌发病密切相关,可能通过调控卵巢癌的脂质代谢从而参与卵巢癌的发生发展,进而影响患者预后。
Abstract
Objective To investigate the expression and clinical significance of ATP binding cassette transporter A1 (ABCA1) in ovarian cancer.Methods Two hundred and twelve patients who were admitted into Beijing Obstetrics and Gynecology Hospital from January 2016 to December 2020 were selected , (106 patients with ovarian cancer as the experimental group and 106 patients with benign ovarian tumor as the control group). Fasting elbow venous blood was collected routinely, and the blood lipid contents (triglyceride (CHO), total cholesterol (TG), high density lipoprotein (HDL) and low-density lipoprotein (LDL)between the two groups were analyzed by enzyme oxidation method; tissue samples were collected immediately after surgical resection. The expression of ABCA1 in tissues was detected by immunohistochemistry and western-blot; Kaplan Meier survival analysis was used to estimate the relationship between ABCA1 expression and patient survival, and log rank method was used for further verification.Results Compared with the control group, the blood lipid levels (CHO, TG, HDL, LDL) in peripheral blood of patients with ovarian cancer were significantly lower (P<0.01); ABCA1 was expressed in ovarian cancer and benign ovarian tumors, and the expression in ovarian cancer (0.15±0.02) was significantly higher than that in the control group (0.06±0.01, P<0.01).Conclusions ABCA1 is closely related with the pathogenesis of ovarian cancer, which may participate in the occurrence and development of ovarian cancer by regulating the lipid metabolism of ovarian cancer, and then affect the prognosis of patients.
关键词
ABCA1 /
卵巢癌 /
脂质代谢 /
发病机制
Key words
ATP binding cassette transporter A1 /
ovarian cancer /
lipid metabolism /
pathogenesis
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基金
首都医科大学附属北京妇产医院院内课题(FCYY201923)
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