目的 探讨甲基化转移酶3(METTL3)在早期鳞状细胞宫颈癌中的表达及其与临床特征和预后的关系。方法 纳入2008年1－12月行广泛性子宫切除术治疗的110例早期宫颈癌石蜡组织标本。采用免疫组织化学检测肿瘤标本和癌旁非肿瘤宫颈组织中METTL3的表达,将患者分为METTL3高表达及低表达组,使用Kaplan-Meier生存分析法明确METTL3表达水平与预后的关系,使用Cox比例风险回归分析早期宫颈癌患者预后不良的影响因素。结果 宫颈癌肿瘤组织METTL3表达水平(2.705±0.273)明显低于正常宫颈标本(8.574±0.421),差异有统计学意义(P<0.01)。METTL3低表达与较高的FIGO分期(P=0.041)、盆腔淋巴结转移(P=0.001)相关。Kaplan-Meier生存分析表明METTL3低表达有低的总生存率(HR=3.576,95% CI:1.474~8.677,P=0.0048)。Cox多因素分析表明,FIGO分期(HR=5.109, 95% CI: 1.891~11.135, P<0.01)、盆腔淋巴结转移(HR=2.395, 95% CI:1.156~4.879, P=0.017)及METTL3低表达(HR=6.187, 95% CI:2.314~21.292, P=0.004)是早期宫颈癌患者的独立预后因素。结论 METTL3 表达水平在宫颈癌组织中明显下降,FIGO分期、盆腔淋巴结转移及METTL3 表达水平是早期鳞状细胞宫颈癌患者预后不良的影响因素。
Abstract
Objective To investigate the relationship between the expression of METTL3 and its clinical features and prognosis in early-stage squamous cell cervical cancer.Methods This study included paraffin samples from 110 patients with cervical cancer who underwent radical hysterectomy from January 2008 to December 2008. Immunohistochemistry was used to detect the METTL 3 expression in tumor specimens and non-tumor cervical tissues, and patients were divided into high-expression group and low-expression group. Kaplan-Meier survival analysis was used to determine the relationship between the expression level of METTL 3 and prognosis. Cox proportional hazard regression analysis was used to analyze the influencing factors of poor prognosis of early cervical cancer patients.Results The expression level of METTL3 in tumor tissues of patients with cervical cancer was significantly lower than that in normal cervical specimens (P<0.01). Low METTL3 expression was associated with higher FIGO staging (P=0.041) and pelvic lymph node metastasis (P=0.001). Kaplan-Meier survival analysis showed that low METTL3 expression was associated with worse overall survival (HR=3.576, 95%CI=1.474-8.677, P=0.0048). Cox multivariate analysis showed that FIGO installment (HR=5.109, 95% CI: 1.891-11.135, P<0.01), pelvic metastases (HR=2.395, 95 % CI: 1.156-4.879, P<0.05),and low expression of METTL3(HR=6.187, 95% CI: 2.314-21.292, P=0.004) were the independent prognostic factor in patients with early cervical cancer.Conclusion METTL3 expression level has decreased significantly in cervical cancer tissue. The FIGO staging, pelvic lymph node metastasis and METTL3 expression levels are independent indicators for poor prognosis of patients with early squamous cell cervical cancer.
关键词
宫颈癌 /
甲基化转移酶3 /
预后 /
生存分析
Key words
cervical cancer /
METTL3 /
prognosis /
survival analysis
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基金
广东省自然科学基金(2021A1515011221)
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