目的 研究电针足三里对失血性休克(HS)海水浸泡损伤延迟静脉复苏大鼠的生存率及脏器功能的影响。方法 选取100只SPF级雄性SD大鼠,按随机数字表法分为HS+海水浸泡组(对照组)和HS+海水浸泡+电针组(电针组),每组50只。大鼠经颈动脉快速放血,失血量为全身血容量的45%。失血后立即将剑突下0.5 cm以下身体在(23±1)℃人工海水中浸泡30 min,海水浸泡完成后2 h,回输全部失血和2倍失血量的乳酸林格液。电针组于浸泡后0.5 h电针双侧足三里,对照组不针刺。监测两组动物的24 h生存率,及失血前,浸泡后即刻,浸泡后2、5、24 h的Cr、CK-MB、ALT及DAO的变化。结果 (1)电针组24 h生存率(90.0%)显著高于对照组(50.0%),差异有统计学意义(χ2=4.494,P=0.034)。(2)浸泡后2 h,电针组的DAO[(9.98±1.00)mg/ml]显著低于对照组[(11.99±0.85)mg/ml],差异有统计学意义(t=4.325,P=0.001)。浸泡后5 h,电针组的CK-MB(501.00±204.94)U/L、ALT(65.38±7.67)mg/ml及DAO(8.37±1.54)mg/ml显著低于对照组,差异有统计学意义(1101.38±336.01,t=4.315,P=0.001;97.64±7.90,t=8.286,P=0.000;10.02±0.91,t=2.367,P=0.035)。浸泡后24 h,电针组的Cr(19.50±4.11)低于对照组(24.88±2.42),差异有统计学意义(t=3.191,P=0.008)。结论 电针足三里能提高HS海水浸泡延迟静脉复苏大鼠的24 h生存率,改善脏器功能,有抗海战伤休克的作用。
Abstract
Objective To study the effects of electroacupuncture at Zusanli(ST36) point on the survival rate and organ function of rats with delayed intravenous resuscitation from seawater immersion injury in hemorrhagic shock (HS). Methods 100SPF-grade male SD rats were selected and divided into HS + seawater immersion group (control group) and HS + seawater immersion + electroacupuncture group (electroacupuncture group) according to the random number table method. The rats were rapidly bled via the carotid artery, and the blood loss was 45% of the total blood volume. Immediately after blood loss, the body below the glabella was immersed in artificial seawater at (23±1) ℃ for 30 min. Blood loss and 2 times the amount of blood loss in lactated Ringer’s solution were returned 2 h after seawater immersion. The electroacupuncture group was acupunctured at 0.5 h after immersion with bilateral ST36, and the control group was not acupunctured. The 24 h survival rate and the changes ofCr, CK-MB, ALT, and DAO before blood loss, immediately after immersion, and 2 h, 5 h, and 24 h after immersion were monitored in both groups. Results The 24h survival rate was significantly higher in the electroacupuncture group (90.0%) than in the control group (50.0%,χ2=4.494,P=0.034). The DAO of the electroacupuncture group(9.98±1.00)was significantly lower than that of the control group 2h after immersion (11.99±0.85,t=4.325,P=0.001). At 5 h after immersion, CK-MB(501.00±204.94), ALT(65.38±7.67), and DAO(8.37±1.54) in the electroacupuncture group were significantly lower than those in the control group (1101.38±336.01,t=4.315, P=0.001;97.64±7.90,t=8.286,P=0.000;10.02±0.91,t=2.367,P=0.035). The Cr(19.50±4.11)in the electroacupuncture group was lower than that in the control group 24 h after immersion (24.88±2.42,t=3.191,P=0.008). Conclusions Electroacupuncture of the ST36 can improve the 24 h survival rate of rats with delayed intravenous resuscitation by seawater immersion following hemorrhagic shock, improve the organ function of animals injured by seawater immersion in hemorrhagic shock, and has the effect of anti-shock.
关键词
电针 /
足三里 /
失血性休克 /
海水浸泡 /
延迟静脉复苏 /
脏器功能
Key words
electroacupuncture /
Zusanli /
hemorrhagic shock /
seawater immersion /
delayed intravenous resuscitation /
organ function
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