目的 探讨菊粉对炎症性肠病(IBD)小鼠肠道炎症的影响及作用机制。方法 40只C57BL/6小鼠随机分为正常对照组、菊粉对照组、模型组和菊粉治疗组(n=10)。菊粉对照组和菊粉治疗组使用菊粉灌胃(500 mg/kg)干预7 d,正常对照组和模型组每天0.9%氯化钠注射液灌胃,干预第8天模型组和菊粉治疗组开始饮用2.5% DSS溶液14 d诱导IBD模型,正常对照组和菊粉对照组饮用蒸馏水。14 d后处死小鼠,分析小鼠一般情况和疾病活动指数(DAI)评分,测量结肠长度,比较结肠组织病理变化,使用实时荧光定量PCR检测结肠组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的mRNA表达,通过蛋白质印记法检测结肠组织中凋亡相关蛋白和核因子-κB(NF-κB)蛋白表达。结果 与正常对照组相比,模型组小鼠一般情况差,DAI评分增高(P<0.05),结肠长度缩短[(6.61±0.33)cm vs.(8.21±0.32) cm,P<0.05)],TNF-α、IL-1β、IL-6的mRNA表达增加(P<0.05),Bcl-2表达下降,Bax、cleaved caspase-3表达增加,NF-κB蛋白表达增加(P<0.05),菊粉对照组无明显差异。与模型组相比,菊粉治疗组一般情况明显改善,DAI评分降低(P<0.05),恢复结肠长度[(7.06±0.31) cm vs.(6.61±0.33) cm,P<0.05],TNF-α、IL-1β、IL-6的mRNA表达下降(P<0.05),Bcl-2表达增加,Bax、cleaved caspase-3表达减少,NF-κB蛋白表达减少(P<0.05)。结论 菊粉可以通过抑制NF-κB进而抑制肠道炎症及细胞凋亡,从而改善IBD小鼠症状。
Abstract
Objective To explore the effect and mechanism of inulin on intestinal inflammation in mice with inflammatory bowel disease (IBD). Methods Forty C57BL/6 mice were randomly divided into normal control group, inulin control group, model group and inulin treatment group (n=10). The inulin control group and inulin treatment group were gavaged 500 mg/kg inulin daily for 14 days, while the normal control group and model group were gavaged 0.9% Sodium Chloride Injection daily. The model group and inulin treatment group were induced IBD models by drinking 2.5% DSS for 7 days on day 8, while the normal control group and the inulin control group were given distilled water. The mice were killed 14 days later. The general condition and disease activity index (DAI) scores were analyzed, the colon length was measured, and the pathological changes of colon tissues were compared. The mRNA expression of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6)in colon tissues were detected by real-time fluorescent quantitative PCR, and the expression of apoptosis-related proteins and NF-κB proteins in colon tissues was detected by western blot. Results Compared with the normal control group, the model group was generally worse, DAI score increased (P<0.05), colon length was shortened [ (6.61±0.33) cm vs. (8.21±0.32) cm, P<0.05], mRNA expressions of TNF-α, IL-1β and IL-6 increased (P<0.05), Bcl-2 protein decreased, Bax, cleaved caspase-3 and NF-κB protein increased (P<0.05), but there was no significant difference in the inulin control group. Compared with model group, the inulin treatment group was significantly improved in general condition, DAI score decreased (P<0.05), colon length recovered [(7.06±0.31) cm vs. (6.61±0.33) cm, P<0.05], mRNA expression of TNF-α, IL-1β and IL-6 decreased (P<0.05), Bcl-2 expression increased, and Bax and cleaved caspase-3 expression decreased (P<0.05). Conclusions Inulin can inhibit intestinal inflammation and cell apoptosis by inhibiting NF-κB , thereby improving the symptoms of IBD mice.
关键词
菊粉 /
炎症性肠病 /
核因子κB /
细胞凋亡
Key words
inulin /
inflammatory bowel disease /
NF-κB /
cell apoptosis
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