目的 探讨尿石素A对高脂饮食老龄小鼠白色脂肪棕色化的影响。方法 老龄小鼠18只随机分为对照组、高脂饮食组(HFD)和尿石素A干预组(UA),每组6只。UA组小鼠予尿石素A灌胃(吐温-80助溶),对照组和HFD组给予等剂量的吐温-80灌胃。实验结束后,记录各组小鼠的体质量和产热量,HE染色观察脂肪组织形态,qRT-PCR和Western blot检测棕色脂肪相关基因解偶联蛋白1(UCP1)、过氧化物酶体增殖体激活受体γ(PPARγ)与过氧化物酶体增殖体激活受体γ共激活因子-1α(PGC-1α)的表达。结果 与对照组相比,HFD组小鼠的体质量明显增加,日间产热量[(0.45±0.03)kcal/h vs.(0.62±0.05)kcal/h]和夜间产热量[(0.49±0.05)kcal/h vs.(0.73±0.06)kcal/h]均显著降低(P<0.05),脂肪细胞体积变大,UCP1、PPARγ与PGC-1α的转录表达降低(P<0.05)。而尿石素A干预降低高脂饮食小鼠的体质量,增加日间产热量[(0.57±0.03)kcal/h vs.(0.45±0.03)kcal/h]和夜间产热量[(0.69±0.07)kcal/h vs.(0.49±0.05)kcal/h],减小脂肪细胞体积,促进UCP1、PPARγ与PGC-1α的转录表达(P<0.05)。结论 尿石素A促进高脂饮食小鼠白色脂肪棕色化。
Abstract
Objective To investigate the effects of urolithin A on browning of white adipose in aged mice fed on a high-fat diet. Methods Eighteen aged mice were randomly divided into control group, high-fat diet (HFD) group and urolithin A administration group (UA), with 6 mice in each group. UA group was given urolithin A (Tween-80 aid solution), control group and HFD group were given the same dose of Tween-80. After the experiment, the body weight and heat production were detected, the morphology of adipocytes was observed by HE staining. qRT-PCR and Western blot were used to investigate mRNA and protein levels of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor γ (PPARγ) and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α). Results Compared with the control group, the body weight of HFD group significantly increased, the heat production of the daytime[(0.45±0.03) kcal/h vs. (0.62±0.05) kcal/h] and nighttime[(0.49±0.05) kcal/h vs. (0.73±0.06) kcal/h] significantly decreased(P<0.05), and the cell size of white adipose tissue increased. Moreover, UCP1, PPARγ and PGC-1α expressions decreased (P<0.05). However, urolithin A intervention reduced the body weight of high-fat diet mice, increased daytime heat production [(0.57±0.03) kcal/h vs. (0.45±0.03) kcal/h] and nighttime heat production [(0.69±0.07) kcal/h vs. (0.49±0.05) kcal/h], and promoted the transcriptional expression of UCP1, PPARγ and PGC-1α expressions (P<0.05). Conclusions Urolithin A can promote the browning of white adipose tissue in aged mice fed on a high-fat diet.
关键词
尿石素A /
肥胖症 /
白色脂肪组织 /
棕色脂肪组织
Key words
urolithin A /
obesity /
white adipose tissue /
brown adipose tissue
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