抗性糊精对慢性不可预知温和应激大鼠肠道菌群的影响

雷静; 张馨予; 陈立军; 王大宁; 孟斌; 温晓昶; 王江平

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武警医学 ›› 2023, Vol. 34 ›› Issue (9) : 794-798.

论著

抗性糊精对慢性不可预知温和应激大鼠肠道菌群的影响

雷静¹, 张馨予¹, 陈立军¹, 王大宁², 孟斌³, 温晓昶¹, 王江平¹

作者信息 +

Effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress

LEI Jing¹, ZHANG Xinyu¹, CHEN Lijun¹, WANG Daning², MENG Bin³, WEN Xiaochang¹, WANG Jiangping¹

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文章历史 +

摘要

目的探究抗性糊精对慢性不可预知温和应激大鼠肠道菌群的影响。方法 24只雄性SD大鼠随机分为对照组、模型组和抗性糊精组,大鼠基于慢性不可预知温和应激(CUMS)建立抑郁模型,3周后进行糖水偏好实验和旷场实验检测。模型组筛选应激敏感大鼠,其他两组随机选择等数量大鼠利用16S rRNA测序技术检测其肠道菌群的组成和结构变化。结果与对照组相比,模型组大鼠肠道菌群厚壁菌门[(0.92251±0.02838)vs.(0.82572±0.00761)]、阿克曼菌属[(0.01327±0.00076)vs.(0.00000±0.00000)]、乳球菌属[(0.00029±0.00010)vs.(0.00000±0.00000)]相对丰度显著降低(P<0.05);拟杆菌门[(0.04671±0.00263)vs.(0.12341±0.03576)]、粪杆菌属[(0.00011±0.00004)vs.(0.00057±0.00005)]、Ruminococcaceae_UCG_014[(0.01003±0.00384)vs.(0.10976±0.04364)]相对丰度显著升高(P<0.05)。与模型组相比,抗性糊精可对丰度发生变化的菌群显著回调(P<0.05)。结论抗性糊精能够调节CUMS大鼠的肠道菌群,通过改善益生菌的相对丰度、降低致病菌的相对丰度促使肠道恢复动态平衡,为抑郁症的治疗提供实验基础研究。

Abstract

Objective To explore the effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress.Methods Twenty-four male SD rats were randomly divided into a control group, a model group and a resistant dextrin group. A depression-like rat model was established using chronic unpredictable mild stress (CUMS). After 3 weeks of stress modelling, sucrose preference test and open- field test were used to evaluate the depression-like behavior of rats. Stress sensitive rats were screened in the model group, and the same number of rats in the other two groups were randomly selected to detect the composition and structural changes of gut microbiota by 16S rRNA sequencing.Results Compared with the control group, relative abundance of Firmlcutes [(0.92251±0.02838) vs. (0.82572±0.00761)], Akkermansia[(0.01327±0.00076) vs. (0.00000±0.00000)]and Lactococcus [(0.00029±0.00010) vs. (0.00000±0.00000)] in the model group was significantly reduced (P<0.05); relative abundance level of Bacteroidetes (0.04671±0.00263) vs. (0.12341±0.03576), Faecalibacterium[(0.00011±0.00004)vs.(0.00057±0.00005)]and Ruminococcaceae_UCG_014 [(0.01003±0.00384)vs.(0.10976±0.04364)] was significantly increased (P<0.05). Compared with the model group, resistant dextrin could significantly callback the microflora with changed abundance (P<0.05).Conclusions Resistant dextrin can regulate the gut microbiota of CUMS rats and restore intestinal dynamic balance by improving the relative abundance of probiotics and reducing the relative abundance of pathogenic bacteria, which provides experimental basis for the treatment of depression.

导出引用

雷静, 张馨予, 陈立军, 王大宁, 孟斌, 温晓昶, 王江平. 抗性糊精对慢性不可预知温和应激大鼠肠道菌群的影响[J]. 武警医学. 2023, 34(9): 794-798

LEI Jing, ZHANG Xinyu, CHEN Lijun, WANG Daning, MENG Bin, WEN Xiaochang, WANG Jiangping. Effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress[J]. Medical Journal of the Chinese People Armed Police Forces. 2023, 34(9): 794-798

中图分类号： R749.4

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