目的 探讨红景天苷对血管生成及骨血管偶联的影响。方法 采用MTS法探究成骨细胞条件培养液对血管内皮细胞增殖活性的影响;采用Transwell小室迁移实验探究成骨细胞条件培养液对血管内皮细胞迁移数量的影响;Matrigel管腔形成实验探究成骨细胞条件培养液对管腔形成长度的影响;胎鼠跖骨血管生成实验用于研究红景天苷对内皮细胞出芽面积的影响。结果 与条件培养液组比较,经红景天苷处理后的条件培养液组细胞活性明显升高,差异均具有统计学意义(P＜0.01);红景天苷组的血管内皮细胞的迁移数量及HUVEC管腔形成长度与条件培养液组相比显著增加,差异有统计学意义(P＜0.01);红景天苷和血管内皮生长因子(VEGF)处理组的内皮细胞出芽面积均明显大于对照组,差异有统计学意义(P＜0.01),且上述作用均能被抗VEGF抗体Avastin阻断。结论 红景天苷是一种较好的促血管生成药物单体,且可通过上调VEGF的表达水平促进骨血管偶联。
Abstract
Objective To study the effect of salidroside (SAL) on angiogenesis and bone vessel coupling. Methods MTS method was used to investigate the effect of osteoblast-conditioned medium on the proliferation activity of vascular endothelial cells. Transwell cell migration assay was used to investigate the effect of osteoblast-conditioned medium on the migration of vascular endothelial cells. Matrigel lumen formation experiment was used to investigate the effect of osteoblast-conditioned medium on lumen formation length. Fetal metatarsal angiogenesis assay was used to evaluate the effect of SAL on the sprouting area of endothelial cells. Results Compared with the conditioned culture medium group, the cell activity of the conditioned culture medium group after SAL treatment significantly increased, and the differences were statistically significant (P<0.01). The number of vascular endothelial cell migration and the length of HUVEC lumen formation in SAL group significantly increased compared with that in conditioned culture medium group, and the difference was statistically significant (P<0.01). The area of endothelial cell budding in SAl and vascular endothelial growth factor (VEGF) treatment groups was significantly larger than that in the control group, with statistical significance (P<0.01), and the above effects could be blocked by the anti-VEGF antibody Avastin. Conclusions SAL is a good angiogenic drug monomer, and can promote bone vascular coupling by up-regulating the expression of VEGF.
关键词
红景天苷 /
血管内皮细胞 /
血管内皮生长因子 /
血管生成 /
骨血管偶联
Key words
salidroside /
vascular endothelial cell /
vascular endothelial growth factor /
angiogenesis /
bone vessel coupling
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