目的 分析北京航天总医院急性呼吸道感染患儿病原体检测情况,为临床诊疗及防治提供依据。方法 回顾性分析2023年9月至11月北京航天总医院儿科门诊就诊的1300例急性呼吸道感染患儿咽拭子标本呼吸道病原体检测结果,采用PCR荧光探针法检测甲型流感病毒(INFA)、乙型流感病毒(INFB)、呼吸道合胞病毒(RSV)、副流感病毒-1(PIV-1)、副流感病毒-3(PIV-3)、腺病毒(ADV)、肺炎支原体(MP)七种病原体RNA。结果 1300例患儿中,总阳性率为54%,病原体核酸阳性率依次为INFA(24.6%)、RSV(10.8%)、MP(9.9%)、ADV(8.3%)、PIV1(2.6%) 、PIV 3(1.3%)、INFB(0.9%),59例(8.4%)存在混合感染,且都是同时检测出2种病原体。不同性别、不同年龄组之间总阳性率差异无统计学意义(P＞0.05)。MP感染在7～14岁组阳性率最高,INFB感染在各年龄组阳性率均较低,但是在4~6岁组最高,RSV及PIV3感染在1～3岁组阳性率高于其他3个年龄组,且差异有统计学意义(P<0.05)。其余病原体检出率各年龄组间比较无差异。结论 该医院2023年秋季就诊急性呼吸道感染患儿病原体以INFA为主,存在混合感染,不同年龄组患儿病原体存在差异。
Abstract
Objective To analyze pathogen detection in children with acute respiratory infection, and to provide evidence for clinical diagnosis, treatment and prevention. Methods The results of respiratory pathogen detection in throat swab samples of 1300 children with acute respiratory infection in the pediatric outpatient department of Beijing Aerospace General Hospital from September to November 2023 were retrospectively analyzed. The RNA of influenza A virus (INFA), influenza B virus (INFB), respiratory syncytial virus (RSV), parainfluenza virus-1 (PIV-1), parainfluenza virus-3 (PIV-3), adenovirus (ADV) and Mycoplasma pneumoniae (MP) were detected by PCR fluorescent probe. Results The total positive rate was 54.0%, and the positive rates of pathogen nucleic acid were INFA (24.6%), RSV (10.8%), MP (9.9%), ADV (8.3%), PIV1 (2.6%), PIV 3 (1.3%) and INFB (0.9%), 59 cases (8.4%) had mixed infection, and both pathogens were detected at the same time. There was no significant difference in the total positive rate between different genders or different age groups (P>0.05). The positive rate of MP infection was the highest in the 7-14 year old group, the positive rate of INFB infection was the highest in the 4-6 year old group, and the positive rate of RSV and PIV3 infection was the highest in the 1-3 year old group, with statistically significant difference (P>0.05). There was no difference in the detection rate of other pathogens among different age groups. Conclusions INFA is the main pathogen in children with acute respiratory infection, there are mixed infections, and there are differences in pathogens among children of different age groups.
关键词
急性呼吸道感染 /
儿童 /
呼吸道病原体 /
核酸检测
Key words
acute respiratory infection /
children /
respiratory pathogen /
nucleic acid detection
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 刘 珍,刘芯如,何春花,等.2010-2016年中国5岁以下儿童死亡率及主要死因分析[J].中华预防医学杂志,2019,53(4):411-414.
[2] Liu W K, Liu Q, Chen D H,et al.Epidemiology of acute respiratory infections in children in Guangzhou: a three-year study [J]. PLoS One, 2014, 9(5): e96674.
[3] 中华医学会儿科分会呼吸学组,重庆市妇幼卫生学会儿童健康专委会,中国医药卫生文化协会疫苗与健康分会. 中国儿童呼吸道合胞病毒感染防控的专家倡议(2023)[J].中华医学杂志,2023,103(40):3155-3159.
[4] 江载芳,申昆玲,沈 颖.诸福棠实用儿科学8版[M]. 北京:人民卫生出版社,2015:1247.
[5] 张 贺,王德兴,冀 虎,等.儿童急性呼吸道感染常见病毒分布及其流行病学特征分析[J].国际病毒学杂志,2023,30(3):239-243.
[6] 徐 亮,邱灿林,徐子强,等. 多重核酸检测系统同时检测22种呼吸道病原体的应用效果研究[J]. 现代医药卫生,2023,39(24):4141-4144.
[7] 赵光华,李炜煊,陈展泽,等. 909例儿童急性呼吸道感染病原体RNA检测结果分析[J]. 海南医学,2020,31(6):744-747.
[8] 李静静,黄建英,刘妙玲,等. 广州地区2017-2022年儿童呼吸道感染疾病负担及常见病原体流行特征[J]. 中国感染控制杂志,2023,22(1):44-51.
[9] 姚 瑶,李爱华,宋文琪.2016-2018年北京地区儿童急性呼吸道感染病原体流行特征分析[J].中华微生物学和免疫学杂志,2019,39(2):88-93.
[10] 袁 青,史大伟,窦海伟,等. 多重PCR技术检测儿童呼吸道病原的结果分析及临床应用价值评估[J]. 中国医药生物技术,2022,17(6):512-517.
[11] 孙 宇,朱汝南,王 芳,等. 2023年春季北京地区儿童急性呼吸道感染病原谱分析[J]. 中华儿科杂志,2024,62(2):159-164.
[12] 葛丽霞,齐美琦,王柏茵,等. 460例急性呼吸道感染患儿病原学分析[J]. 首都公共卫生,2023,17(1):11-14.
[13] Wang H,Zheng Y,Deng J,et al. Prevalence of respiratory viruses among children hospitalized from respiratory infections in Shenzhen,China[J].Virol J,2016,13:39.
[14] Li Z J,Zhang H Y,Ren L L,et al. Etiological and epidemiological features of acute respiratory infections in China[J].Nat Commun,2021,12(1):5026.
[15] 朱 云,卢 根,靳 蓉,等. 2017-2020年我国儿童急性下呼吸道感染住院病例中呼吸道合胞病毒的感染情况分析[J]. 中华预防医学杂志,2022,56(12):1739-1744.
[16] 王 丹,孙树荣,曲 芬. 北苑地区儿童冬春季社区获得性肺炎非细菌性病原体流行病学分析[J]. 标记免疫分析与临床,2021,28(3):402-405.
[17] 谢正德,邓继岿,任丽丽,等. 儿童呼吸道感染病原体核酸检测专家共识[J]. 中华实用儿科临床杂志,2022,37(5):321-332.
[18] 顾秀丽,姚 瑶,田晓怡,等. 2020年北京地区儿童常见呼吸道病原体流行情况分析[J]. 中华微生物学和免疫学杂志,2022,42(2):141-147.
[19] 王 丽,于玉梅,邓清秀.儿童社区获得性肺炎的病原体分布及临床特征分析[J].实用心脑肺血管病杂志,2022,30(8):85-90.
[20] 叶 青,王笑灵,田国保,等. 儿童及成人重症社区获得性肺炎病原学及流行病学研究[J].临床和实验医学杂志,2019,18(11):1169-1172.
基金
北京市海淀医院科研基金项目(KYY2024006);北京航天总医院创新基金科研立项(2023-720)
PDF(945 KB)
