目的 探讨血竭、青黛单独及配伍对糖尿病大鼠皮肤创面组织愈合的效果及作用机制。方法 制备糖尿病大鼠模型,制备皮肤缺损后随机分成正常对照组、模型对照组、血竭单药组、青黛单药组和血竭-青黛配伍组,给药14 d后,脱颈椎处死,计算皮肤创面愈合率,HE染色观察皮肤组织病理变化;实时荧光定量 PCR 技术检测TGF-β1/Smads信号通路相关因子表达。结果 与模型对照组相比,青黛单药组皮肤创面愈合率低,血竭、血竭-青黛配伍组愈合率显著提升(64.19%,61.16%)。HE染色病理观察提示,血竭、血竭-青黛配伍组的皮肤组织新生表皮层较厚,有较多的新生毛细血管及少量炎性细胞浸润,表皮完整性优于青黛单药组。与模型对照组比较,各干预组TGF-β1 mRNA表达升高(P<0.05);血竭单药组Smad2 mRNA(5.204±1.070)、Smad3 mRNA(1.201±0.052)表达升高;血竭、青黛单用上调Smad 7 mRNA表达[(5.125± 1.918)、(6.896±3.628)],配伍后表达下调(2.814± 0.285);血竭、青黛单药组的ERK1/2表达与模型组差异不显著,但二者配伍后表达水平(1.005± 0.041)低于模型组(P<0.05);青黛单药组的JNK mRNA(5.766±0.703)、p-38MAPK mRNA(3.664±0.357)较模型组表达升高(P<0.05)。结论 血竭、青黛单药及配伍有促进糖尿病大鼠模型皮肤创面愈合的作用,其机制可能与调控TGF-β1/Smads信号通路相关因子表达相关。
Abstract
Objective To investigate the effect and mechanism of Xuejie and Qingdai and in combination on skin wound healing in diabetes rats. Methods The diabetic rat model was prepared and randomly divided into normal control group, model control group, Xuejie group, Qingdai group and Xuejie-Qingdai compatibility group, with 6 rats in each group. After 14 days of administration, the cervical vertebrae were removed for execution, and the healing rate of the wounds was calculated, and H-E staining was used to observe the pathology of the skin; RT-PCR was used to detect the expression of the factors related to the TGF-β1/Smads signaling pathway. Results Compared with the model control group, the Qingdai group had a lower skin wound healing rate, while the Xuejie group and combination group showed a significant improvement in healing rate (64.19% and 61.16%). The pathological observation of HE staining suggested that the epidermal integrity of the skin tissues in the Xuejie group and the combination group were better than that the Qingdai group. The newly formed epidermal layer was thicker, the cell arrangement was irregular, and there were more newly formed capillaries and a small amount of inflammatory cell infiltration. Compared with the model control group, the expression of TGF-β1 mRNA increased in each intervention group (P<0.05); The expression of Smad2 and Smad3 mRNA in the Xuejie group (5.204±1.070, 1.201±0.052)was higher than that in the model control group; the expression level of Smad 7mRNA was upregulated by using only Xuejie or Qingdai(5.125±1.918, 6.896±3.628), while its expression was downregulated by their combination[(5.125±1.918), (6.896±3.628)]; the expression of ERK1/2 in the Xuejie and Qingdai monotherapy groups were not significantly different from that in the model group, but there was statistically significant difference between the combination group(3.664±0.357) and the model group (P<0.05). The expression of JNK and p-38MAPK mRNA in the Qingdai group was higher than the model group (P<0.05). Conclusions Xuejie and Qingdai can promote skin wound healing in diabetes rats, and the mechanism may be related to regulating the expression of TGF-β 1/Smads signal pathway related factors.
关键词
血竭 /
青黛 /
创面愈合 /
TGF-β/Smads
Key words
Xuejie /
Qingdai /
wound healing /
TGF-β/Smads
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