爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响

李梦; 李爽; 张欣然; 朱彪; 郑雪; 王坚

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武警医学 ›› 2024, Vol. 35 ›› Issue (5) : 395-398.

论著

爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响

李梦¹, 李爽², 张欣然³, 朱彪², 郑雪², 王坚¹

作者信息 +

Effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats

LI Meng¹, LI Shuang², ZHANG Xinran³, ZHU Biao², ZHENG Xue², WANG Jian¹

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文章历史 +

摘要

目的探讨爱帕琳肽13(Apelin-13)对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响。方法分离、培养GK糖尿病大鼠BMSCs。成骨诱导培养后,检测碱性磷酸酶(ALP)活性,Von Kossa染色观察钙结节矿化程度,O2K线粒体呼吸仪测定不同状态下线粒体呼吸功能。结果 Apelin-13增强GK糖尿病大鼠BMSCs的ALP活性;促进钙结节矿化;与GK组比较,Apelin-13有效提高两种状态下线粒体呼吸功能:复合物Ⅰ态3呼吸[(16.1±1.8) vs. (5.6±0.9)]pmol/(s×ml),复合物Ⅰ+复合物Ⅱ态3呼吸[(71.1±8.6) vs.(49.0±4.8)] pmol/(s×ml)。结论 Apelin-13可促进GK大鼠BMSCs骨向分化,并提高其线粒体呼吸功能。

Abstract

Objective To investigate the effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats. Methods The BMSCs of GK diabetic rats were extracted. After cultured in osteogenic differentiation medium, the activity of alkaline phosphatase (ALP) was detected. Von Kossa staining was used to evaluate calcium nodules formation, and the mitochondrial respiratory function under different states was determined by O2K mitochondrial respiration apparatus. Results Apelin-13 enhanced the ALP activity of BMSCs in GK diabetic rats, and promoted calcium nodules mineralization. Compared with the GK group, Apelin-13 effectively improved mitochondrial respiratory function in two states: complex I state 3 respiration (16.1±1.8) vs. (5.6±0.9)] pmol/(s×ml) and complex Ⅰ+ complex Ⅱ state 3 respiration[(71.1±8.6) vs. (49.0±4.8)] pmol/(s×ml). Conclusions Apelin-13 can promote osteoblast differentiation and enhance mitochondrial respiration function of BMSCs derived from GK diabetic rats.

导出引用

李梦, 李爽, 张欣然, 朱彪, 郑雪, 王坚. 爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响[J]. 武警医学. 2024, 35(5): 395-398

LI Meng, LI Shuang, ZHANG Xinran, ZHU Biao, ZHENG Xue, WANG Jian. Effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats[J]. Medical Journal of the Chinese People Armed Police Forces. 2024, 35(5): 395-398

中图分类号： R780.2

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