目的 探讨髓磷脂蛋白零样蛋白1(MPZL1)对膀胱癌T24细胞增殖、迁移和侵袭能力的影响。方法 通过siRNA转染敲低膀胱癌T24细胞中MPZL1的表达,构建抑制MPZL1表达的膀胱癌细胞实验模型,将细胞分成si-NC的对照组和si- MPZL1实验组。通过ualcan数据库在线分析MPZL1基因在TCGA数据库膀胱癌患者中表达情况。CCK-8实验和平板克隆实验检测膀胱癌细胞增殖的变化,划痕实验和Transwell实验检测膀胱癌细胞迁移和侵袭的变化。结果 MPZL1基因在膀胱癌患者中高表达,且随着临床分期的进展,其表达量呈现上升趋势,MPZL1基因高表达与膀胱癌的生存时间缩短有明显相关性。抑制MPZL1表达可抑制膀胱癌T24细胞的增殖、迁移和侵袭能力。结论 敲低膀胱癌T24细胞中MPZL1表达,可抑制膀胱癌细胞的增殖、迁移和侵袭能力。
Abstract
Objective To investigate the effects of myelin protein zero like 1 (MPZL1) on proliferation, migration and invasion of bladder cancer T24 cells. Methods The expression of MPZL1 in bladder cancer T24 cells was knocked down by siRNA transfection to form an experimental model of bladder cancer cells that inhibited the expression of MPZL1. The cells were divided into si-NC group and si-MPZL1 group. The expression of MPZL1 gene in bladder cancer patients in TCGA database was analyzed online through ualcan database. The changes of cell proliferation were detected by CCK-8 assay and plate cloning assay, and the changes of cell migration and invasion were detected by scratch assay and Transwell assay. Results The MPZL1 gene was highly expressed in patients with bladder cancer and the expression level showed an increasing trend with the progress of clinical stage. The high expression of MPZL1 gene was significantly correlated with the shortened survival time of bladder cancer. Inhibition of MPZL1 expression could effectively inhibit the proliferation, migration and invasion of bladder cancer T24 cells. Conclusions Knocking down the expression of MPZL1 in bladder cancer T24 cells can inhibit the proliferation, migration and invasion of bladder cancer cells.
关键词
膀胱癌 /
髓磷脂蛋白零样蛋白1 /
增殖 /
迁移 /
侵袭
Key words
bladder cancer /
myelin protein zero like 1 /
proliferation /
migration /
invasion
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参考文献
[1] Antoni S, Ferlay J, Soerjomataram I, et al. Bladder Cancer Incidence and Mortality: A Global Overview and Recent Trends[J].Eur Urol,2017,71(1):96-108.
[2] Zhao R, Zhao Z J. Identification of a variant form of PZR lacking immunoreceptor tyrosine-based inhibitory motifs [J]. Biochem Biophys Res Commun, 2003, 303(4): 1028-1033.
[3] Zhao Z J, Zhao R. Purification and cloning of PZR, a binding protein and putative physiological substrate of tyrosine phosphatase SHP-2 [J]. J Biol Chem,1998, 273(45): 29367-72.
[4] Jia D, Jing Y, Zhang Z, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma [J]. Cell Res, 2014, 24(2): 204-17.
[5] 林凤娟,葛潇潇,吴 峥,等.胃癌中MPZL1的表达及其对胃癌细胞增殖能力的影响[J].肿瘤防治研究, 2021, 48(7):7.
[6] Hyuna S, Jacques F, Rebecca L S, et al.Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J].CA Cancer J Clin,2021,71(3):209-249.
[7] Han B, Zheng R, Zeng H, et al. Cancer incidence and mortality in China, 2022 [J]. J Natl Cancer Cent,2024,4(1):213-218.
[8] Lopez-Beltran A, Cookson M S, Guercio B J, et al.Advances in diagnosis and treatment of bladder cancer [J].BMJ,2024,12:e076743.
[9] Ibrahim J, Sean O, Laura B, et al. Epidemiology of bladder cancer in 2023: a systematic review of risk factors [J]. Eur Urol,2023,84(2):176-190.
[10] Lars D, Donna H, Jason E, et al.Bladder cancer [J]. Nat Rev Dis Primers,2023,26(1):58.
[11] Zhao ZJ, Zhao R.Purification and cloning of PZR, a binding protein and putative physiological substrate of tyrosine phosphatase SHP-2[J]. J Biol Chem,1998,273(45):29367-72.
[12] Zhao R, Zhao Z J. Dissecting the interaction of SHP-2 with PZR, an immunoglobulin family protein containing immunoreceptor tyrosine-based inhibitory motifs [J]. J Biol Chem, 2000, 275(8): 5453-5459.
[13] Yeh Y T, Dai H Y,Chien C Y. Amplification of MPZL1/PZR gene in hepatocellular carcinoma. Hepatobiliary Surg Nutr [J]. 2014,3(2): 87-90.
[14] Alice B, François J M, Nicolas B. MPZL1 forms a signalling complex with GRB2 adaptor and PTPN11 phosphatase in HER2-positive breast cancer cells [J]. Sci Rep,2017,14,7(1):11514.
[15] Feng J, Ouyang H, Wang J,et al. MPZL1 upregulation promotes tumor metastasis and correlates with unfavorable prognosis in non-small cell lung cancer [J]. Carcinogenesis,2022,43(10):919-929.
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