目的 了解乳腺癌临床病理特征和血管生成拟态(VM)及CD8+T细胞表达情况的关系,探索VM与肿瘤微环境(TME)的相关性。方法 60例乳腺癌组织标本制备成组织芯片,应用CD31/PAS双重染色法检测VM表达情况,应用免疫组织化学染色法(IHC)计数CD8+T细胞的数量并计算表达率,分析乳腺癌临床病理特征与两者的关系,了解VM与CD8+T细胞的相关性。结果 60例乳腺癌组织样本中VM阳性检出24例(40%),病理分级G3的VM阳性检出率(63.2%)高于G1-2(29.3%),差异有统计学意义(χ2＝6.213,P=0.013)。Ⅲ期VM阳性检出率(54.5%)高于Ⅰ期(16.7)和Ⅱ期(45.0%),差异有统计学意义(χ2＝6.231,P=0.044);三阴型VM阳性表达(60.0%)高于其他分子分型(12%),差异有统计学意义(χ2＝14.0,P＜0.001);浸润性导管癌与其他组织分型在VM检出率上的差异无统计学意义;年龄≥40岁与＜40岁的人群在VM检出率上差异无统计学意义。CD8+T细胞阳性表达检出26例(43.3%),三阴型乳腺癌(TNBC)中CD8+T细胞阳性表达(28.6%)低于其他分子分型(64.0%),差异有统计学意义(χ2＝7.454,P=0.006);年龄、组织分型、病理分级、临床分期在CD8+T细胞表达上的差异无统计学意义。全样本中VM阴性和阳性在CD8+T细胞表达与否上的差异有统计学意义(χ2＝11.584,P＜0.001),但在TNBC样本中差异无统计学意义。结论 VM形成在分期晚和分化差的乳腺癌中更为多见,尤其在TNBC中比例更高,CD8+T细胞更多见于非TNBC的人群。有显著VM形成的乳腺癌中,CD8+ T淋巴细胞往往较少。
Abstract
Objective To understand the relationship between the clinicopathological features of breast cancer and the expression of vasculogenic mimicry (VM ) and CD8+T cells. Futher to explore the correlation between VM and immune microenvironment. Methods 60 cases of breast cancer tissue samples were collected to make tissue chip. The expression of VM was detected by CD31/PAS double staining. IHC was used to assess the expression of CD8+T cells.The relationship among VM expression, CD8+T cell expression and clinicopathological characteristics were analyzed, so as to infer the relationship between VM and CD8+T cells. Results Among 60 cases of breast cancer tissue samples, VM was detected in 24 cases (40%). The positive rate of VM in pathlogical grade G3 was 63.2%, while it was 29.3% in tissues from G1 and G2. The difference was statistically significant (χ2=6.213,P=0.013).The positive detection rate of VM in stage Ⅲ (54.5%) was significantly higher than that in stage Ⅰ/Ⅱ (χ2=6.231,P=0.044). The positive expression of VM in triple negative type was significantly higher than that in other molecular types, and the difference was statistically significant (χ2=14.0,P＜0.001).There was no significant difference in the detection rate of VM with age and histological types.The positive expression of CD8+T cells was detected in 26 cases (43.3%). The positive expression of CD8+T cells in triple negative breast cancer (TNBC) was significantly lower than that in other molecular subtypes, and the difference was statistically significant (χ2=7.454,P=0.006). There was no significant difference in the expression of CD8+ T cells among age, histological type, pathological grade and clinical stage. There was a significant difference in the expression of CD8+ T cells between VM negative and positive in the whole samples (χ2=11.584,P＜0.001), but there was no significant difference in TNBC samples (χ2=1.313,P=0.252). Conclusion VM formation is more common in advanced and poorly differentiated breast cancer, especially in TNBC, and CD8+T cells are more common in non-TNBC. In breast cancer with significant VM formation, CD8+T lymphocytes are often less.
关键词
乳腺癌 /
血管生成拟态 /
肿瘤免疫微环境 /
CD8+T淋巴细胞
Key words
breast cancer /
vasculogenic mimicry /
tumor immune microenvironment /
CD8+T lymphocytes
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基金
国家自然科学基金项目(82002803);陕西省重点研发计划(2020SF-029);吴阶平医学基金项目(320.6750.2021-10-84)
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