目的 探讨卵裂期不同氧浓度对体外受精胚胎发育及妊娠结局的影响。方法 回顾性分析2022-01至2024-03在武警特色医学中心生殖医学科行新鲜囊胚移植的患者的资料,本研究共对156例患者的158个周期进行分析,根据卵裂期胚胎氧气浓度不同分为高氧组(n=53,20% O2共53个周期)和低氧组(n=103,5% O2共105个周期)。比较两组间胚胎发育情况及妊娠结局。结果 两组患者总受精率、正常受精率、卵裂率、D3的优质胚胎率、β-HCG阳性率、临床妊娠率、种植率、流产率及活产率等差异均无统计学意义。低氧组D3可利用胚胎率显著高于高氧组,差异具有统计学意义(72.98% vs 67.90%,P＜0.05)。低氧组囊胚形成率(71.20% vs 79.57%)及可利用囊胚形成率(66.03% vs 74.30%)显著低于高氧组,差异均具有统计学意义(P＜0.05)。结论 卵裂期低氧浓度可以提高D3可利用胚胎率,但不能改善体外受精-胚胎移植胚胎妊娠结局。
Abstract
Objective To investigate the impact of oxygen concentrations during the cleavage stage on IVF embryo development and pregnancy outcomes. Methods This retrospective study analyzed 158 fresh blastocyst transfer cycles performed at the Reproductive Medicine Center of the Characteristic Medical Center of Chinese People’s Armed Police Force between January 2022 and March 2024. Cycles were divided into two groups based on oxygen concentration during cleavage-stage culture: low-oxygen (5% O2, n=103) and high-oxygen (20% O2, n=53) groups. Embryo development parameters and clinical outcomes were compared between groups. Results No statistically significant differences were observed between groups in total fertilization rate, normal fertilization rate, cleavage rate, day-3 high-quality embryo rate, β-HCG positivity rate, clinical pregnancy rate, implantation rate, miscarriage rate, or live birth rate. The low-oxygen group demonstrated significantly higher day-3 usable embryo rate compared to the high-oxygen group(72.98% vs 67.90%,P＜0.05). Conversely, the low-oxygen group showed significantly lower blastocyst formation rate (71.20% vs 79.57%,P＜0.05)and usable blastocyst rate (66.03% vs 74.30%, P＜0.05). Conclusion While low oxygen concentration during cleavage stage improves day-3 usable embryo rate, it does not enhance pregnancy outcomes in IVF-ET cycles.
关键词
氧浓度 /
体外受精 /
胚胎发育 /
囊胚移植 /
妊娠结局
Key words
oxygen concentration /
in vitro fertilization /
embryo development /
blastocyst transfe /
clinical outcome
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