目的 探讨耳针联合越鞠丸对抑郁大鼠的行为学、脑源性神经营养因子（BDNF）、络氨酸激酶B（TrkB）、反应元件结合蛋白（CREB）、可溶性环氧化酶（sEH）和大鼠海马体的影响。方法 取50只SD大鼠,其中10只作为空白对照组（A组）,剩余40只建立抑郁大鼠模型后随机分为4组,即模型组（B组）、耳针组（C组）、越鞠丸组（D组）和耳针联合越鞠丸组（E组）,每组10只。分别于造模后及干预后进行行为学实验评估大鼠抑郁状态。ELISA检测试剂盒测定血浆BDNF、sEH。苏木素-伊红（HE）染色观察大鼠海马形态学变化。通过Real-time PCR和Western blot检测大鼠海马组织中BDNF,TrkB,CREB 含量。结果 造模后B、C、D、E组体重明显降低,糖水偏嗜率明显降低,行为学实验不动时间明显增加,HE 染色结果显示抑郁大鼠海马神经元结构损伤,BDNF,TrkB,CREB mRNA 及蛋白表达降低,差异均有统计学意义（P<0.05）：与B组比较,C、D、E组大鼠体重明显升高,大鼠糖水偏嗜率明显升高,不动时间明显减少,海马神经元结构明显复原,BDNF,TrkB,CREB mRNA 及蛋白表达增加,差异均有统计学意义（P<0.05）。结论 耳针联合越鞠丸能改善抑郁大鼠的运动能力、运动意愿、学习能力、空间记忆能力,同时为开发新的抑郁症诊断工具提供实验依据。
Abstract
Objective To investigate the effects of auricular acupuncture combined with Yueju Pills on the behavior, brain-derived neurotrophic factor （BDNF）, tyrosine kinase B （TrkB）, cAMP response element binding protein （CREB）, soluble epoxide hydrolase （sEH） and hippocampus of depressed rats. Methods A total of 50 SD rats were selected,ten of which were used as blank control group A, and the remaining 40 rats were established as depression rat models and randomly divided into 4 groups,namely a model group (group B),an auricular acupuncture group (group C),a Yueju pill group (group E) and an auricular acupuncture combined with Yueju pill group (group E) , with 10 rats in each group . Behavioral experiments were conducted respectively after modeling and intervention to evaluate the depressive state of rats. Plasma BDNF and sEH were measured by ELISA kit. Hematoxylin-eosin staining (HE) was used to observe the morphological changes of rat hippocampus. The contents of BDNF,TrkB and CREB in hippocampus of rats were detected by real-time PCR and Western blot. Results After modeling,the body weight of group B, C, D and E significantly decreased,the sucrose preference rate significantly decreased,and the immobility time of behavioral experiment significantly increased. The results of HE staining showed that the structure of hippocampal neurons in depressed rats was damaged,and the expression of BDNF,TrkB and CREB mRNA and protein significantly decreased （P<0.05）. Compared with group B,the body weight of the rats in groups C, D and E significantly increased, the sucrose preference rate of the rats significantly increased,the immobility time was significantly reduced, the structure of hippocampal neurons was significantly restored,and the expression of BDNF,TrkB,CREB mRNA and protein was increased. The differences were all statistically significant（P<0.05）. Conclusion The study confirmed the antidepressant effect of auricular acupuncture and Yueju Pills,and clarified the superiority of auricular acupuncture combined with Yueju pills in improving the exercise ability,exercise willingness,learning ability and spatial memory ability of depressed rats. It provides experimental basis for the development of new diagnostic tools for depression.
关键词
耳针 /
越鞠丸 /
抑郁大鼠
Key words
auricular acupuncture /
Yueju pill /
depression rat
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