目的 研究生脉散（SMS）对2型糖尿病大鼠（T2DM）心肌细胞的保护作用。方法 50只性成熟SD雄性大鼠按照随机数字表法分为5组,分别是对照组、T2DM组、SMS干预组[（0.9 g/（kg·d）、1.8 g/（kg·d）、3.6 g/（kg·d）],每组10只。糖尿病大鼠造模成功后,T2DM组给予灌胃生理盐水,SMS组灌胃不同浓度的SMS[0.9 g/（kg·d）、1.8 g/（kg·d）、3.6 g/（kg·d）],连续进行4周。最后一次灌胃后禁食8 h后,异氟烷吸入麻醉后称量大鼠体重,剖开腹部抽取腹主动脉血检测相关指标空腹血糖（FBG）、餐后2 h血糖（2hPBG）、空腹血浆胰岛素（FINS）、胰岛素抵抗指数（HOMA-IR）、胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）,快速取出心脏一部分放于多聚甲醛中进行病理学检测[HE染色、Masson染色、PAS染色、TUNEL染色、免疫荧光染色（Bax、Bcl-2）],另一部分放于-80°C冰箱中以待检测Bax、Bcl-2蛋白水平与acitiviy-9/3活力。结果 与对照组相比,T2DM组FBG、2hPBG、FINS、HOMA-IR、TG、TC、LDL-C升高（P<0.05）,给予SMS干预后降低（P<0.05）;与对照组相比,T2DM组HDL-C降低（P<0.05）,给予SMS干预后升高（P<0.05）;与对照组相比,T2DM组心肌损伤程度（HE染色）、纤维化程度（Masson染色）、糖原沉积度（PAS染色）、凋亡程度（TUNEL染色）、acitiviy-9/3活力升高（P<0.05）,给予SMS干预后降低（P<0.05）;与对照组相比,T2DM组Bax表达（免疫荧光与WB）升高（P<0.05）,给予SMS干预后降低（P<0.05）,而Bcl-2表达（免疫荧光与WB）降低（P<0.05）,给予SMS干预后升高（P<0.05）。结论 SMS能通过改善T2DM大鼠心肌组织的凋亡,进而保护心肌细胞。
Abstract
Objective To investigate the protective effect of Shengmaisan (SMS) on myocardial cells of type 2 diabetic（T2DM）rats. Methods Fifty sexually mature male SD rats were randomly divided into five groups according to the random number table method：control group,T2DM group,Shengmaisan intervention group [0.9 g/（kg·d）, 1.8 g/(kg·d), 3.6 g/(kg·d）], with 10 rats in each group. After successful establishment of diabetes in the rats,the T2DM group was given normal saline by gavage,and the Shengmaisan groups were given different concentrations of Shengmaisan [0.9 g/（kg·d）, 1.8 g/(kg·d), 3.6 g/(kg·d）] by gavage for 4W. After fasting for 8h after the last gavage, the weight of rats was weighed after isoflurane inhalation anesthesia, and the abdominal aorta blood was drawn by laparotomy to detect the relevant indicators （FBG, 2-hour postprandial blood glucose （2hPBG）, fasting plasma insulin （FINS）, insulin resistance index （HOMA-IR）, total cholesterol （TC）, total triglycerides （TG）, low-density lipoprotein cholesterol （LDL-C）, high-density lipoprotein cholesterol （HDL-C））. The heart was quickly removed and put into paraformaldehyde （HE staining, Masson staining, PAS staining, TUNEL staining, immunofluorescence staining （Bax, bcl-2）） and dry ice zone detection （Bax, bcl-2, and activity of caspase-9/3）. Results Compared with the control group, FBG, 2hpbg, FINS, HOMA-IR, TG, TC,and LDL-C increased in T2DM group （P<0.05）, and decreased after intervention with Shengmaisan （P<0.05）; HDL-C in T2DM group decreased（P<0.05）, and increased after the intervention of Shengmaisan （P<0.05）; the degree of myocardial injury （HE staining）, fibrosis （Masson staining）, glycogen deposition （PAS staining）, and apoptosis （TUNEL staining and activity of caspase-9/3） in T2DM group increased （P<0.05）, and decreased after the intervention of Shengmaisan （P<0.05）;the expression of Bax （immunofluorescence and WB） in T2DM group increased （P<0.05）, and decreased after the intervention of Shengmaisan （P<0.05） while the expression of Bcl-2 （immunofluorescence and WB） decreased （P<0.05）,and increased after the intervention of Shengmaisan （P<0.05）. Conclusions Shengmaisan can protect myocardial cells by improving apoptosis of myocardial tissue in T2DM rats.
关键词
生脉散 /
大鼠 /
T2DM /
心肌 /
凋亡
Key words
Shengmaisan /
rat /
T2DM /
myocardium /
apoptosis
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参考文献
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基金
中国博士后面上项目（2022MD713765）
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